For up-to-date licensing information and product-specific disclaimers, see the respective handbook or user manual. Assays for veterinary use only. Regulatory requirements vary by country, product may not be available in your geographic area. PCR and ELISA kits availability/distribution: Outside the U.S. and Canada
Veterinary diagnosticians worldwide rely on INDICAL‘s molecular solutions for Bovine Viral Diarrhea Virus (BVDV) control, using it to test millions of calves annually. Building upon 15 years of experience, we have developed the new virotype BVDV 2.0 RT-PCR Kit (FLI-C 112) with increased ease of use and enhanced protocols.
Test with our best – virotype BVDV 2.0 RT-PCR Kit
- Faster time to result
RT-qPCR runtime of ~1 hour* - Gold standard sensitivity and specificity
Next-generation triplex real-time RT-PCR assay for reliable identification of all known BVDV genotypes
- Additional internal controls for greater confidence
Endogenous Internal Control (β-actin) and Exogenous Internal Control - Increased ease of use
Ready-to-use reagents including a single reaction mix, saving precious lab time - Multiple validated sample types
Blood, plasma, serum, tissue, milk, and ear tissue from cattle - Extended pooling options The high sensitivity of the virotype BVDV 2.0 RT-PCR Kit permits blood samples (whole blood, plasma, serum) and milk samples to be tested in pools consisting of up to 100 individual samples, and ear tissue samples in pools of up to 25 individual samples.**
* See virotype BVDV 2.0 RT-PCR Kit Handbook for detailed information. ** The high sensitivity of the virotype BVDV 2.0 RT-PCR Kit permits blood samples (whole blood, plasma, serum) and milk samples to be tested in pools consisting of up to 100 individual samples, and ear tissue samples in pools of up to 25 individual samples. Pool recommendations and regulations may vary between countries.
| Pathogen type | Virus |
| Species | Ruminants |
| Product type | Ready-to-use assay |
| Pathogen investigated | Bovine Viral Diarrhea Virus (BVDV) |
| Technology | RT-qPCR |
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Handbooks
Handbook virotype BVDV 2.0 RT-PCR Kit (July 2022, EN)
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Handbooks
Handbuch virotype BVDV 2.0 RT-PCR Kit (Juli 2022, DE)
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Brochures
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Handbooks
Instrukcja obsługi virotype BVDV 2.0 RT-PCR Kit (Lipiec 2022, PL)
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Handbooks
Manuel virotype BVDV 2.0 RT-PCR Kit (Julliet 2022, FR)
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Brochures
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Handbooks
Manual virotype BVDV 2.0 RT-PCR Kit (Julio 2022, ES)
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| Certificates of Analysis (CoA) |
Request a Certificate of Analysis and see the quality control data for your product. |
| Safety Data Sheets |
Safety Data Sheets (SDS) for product components are available on request. |
| 1. Maes R, Bronson-Doherty P, Chumbley M, Herrmann DC, Wise A, Kull-Mason S, Miller V, Schroeder C (2017): Detection of Bovine Viral Diarrhea (BVD) Virus in Ear Skin Tissue Samples: Evaluation of a Combination of QIAGEN MagAttract 96 cador Pathogen Extraction and virotype BVDV RT-PCR-based Amplification, 16th Annual Meeting of the Canadian Animal Health Laboratorians Network (CAHLN), June 4-7, 2017, University of Guelph, Guelph, ON, Canada |
| 2. Bauermann FV, Falkenberg SM, Vander Ley B, Decaro N, Brodersen BW, Harmon A, Hessman B, Flores EF, Ridpath JF (2014): Generation of Calves Persistently Infected with HoBi-Like Pestivirus and Comparison of Methods for Detection of These Persistent Infections, Journal of Clinical Microbiology, Volume 52 No. 11 p. 3845–3852 |
| 3. Hoffman B, Depner K, Schirrmeier, H, and Beer, M (2006): A universal heterologous internal control system for duplex real-time RT-PCR assays used in a detection system for pestiviruses. J. of Virological Methods, Volume 136, Issues 1–2, pp.200–209. |
| 4. Fux, R., and Wolf, G. (2012): Transient elimination of circulating bovine viral diarrhoea virus by colostral antibodies inpersistently infected calves: a pitfall for BVDV-eradication programs? Veterinary Microbiology 161 13–19. |
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