Cat. No.: AED-KIT


For detection of IgG antibodies to Anaplasma phagocytophilum (anaplasmosis) in dog serum and plasma samples (Availability/distribution: Outside the U.S. and Canada) [Read more]

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The ANAPLASMA-ELISA DOG is an indirect enzyme immunoassay for detecting IgG antibodies to Anaplasma phagocytophilum in serum and plasma samples from dogs.

Anaplasma phagocytophilum is the causative agent of anaplasmosis - a common tick-borne disease in dogs. In general antibodies to Anaplasma phagocytophilum are detectable 10 to 14 days post infection (2 to 5 days after the morulae are detectable in dyed blodd smears).
A remarkable rise of antibodies within seven through 21 days is characteristic of an active Anaplasma infection. But a high-level antibody titre persisting for several months does not point out an acute anaplasmosis. If there is suspicion of anaplasmosis then besides the clinical symptoms, the dynamic titre should be monitored.

Features and benefits

  • Faster and easier to use compared to Indirect Immunofluorescence Antibody Tests (IFATs) for anaplasmosis
  • High sensitivity of 92.5% and high specificity of up to 97%
    (validated with n = 154 characterized dog sera)

    Improve your workflow efficiency with AFOSA assays

      • Ready-to-use reagents (Positive/Negative Controls, Conjugate, Substrate, Stop Solution)
      • Color-coded caps
        Interchangeable reagents between AFOSA assays
      • Microplate with 12 individual strips of 8 wells
      • All AFOSA ELISA kits use the same short protocol
      • Unified pipetting volume of 100 µl 
      • Suitable for automated ELISA instruments

      For up-to-date licensing information and product-specific disclaimers, see the respective handbook or user manual. Assays for veterinary use only. Regulatory requirements vary by country, product may not be available in your geographic area. PCR and ELISA kits availability/distribution: Outside the U.S. and Canada

      Product picture:

      Pathogen type Bacteria
      Product contents 1 Microtiter plate with 96 wells (coated with Anaplasma antigen), Sample dilution buffer, Wash buffer (10x concentrate), Conjugate solution, Substrate solution, Stop solution, Positive control serum, Negative control serum
      Product type Ready-to-use assay
      Species Dog
      Pathogen investigated Anaplasma phagocytophilum (Anaplasmosis)
      Technology ELISA
      Manufacturer afosa GmbH - part of INDICAL
      Handbook ANAPLASMA-ELISA DOG (December 2022, EN)
      Handbuch ANAPLASMA-ELISA DOG (Dezember 2022, DE)
      Certificates of Analysis (CoA)

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      Safety Data Sheets

      Safety Data Sheets (SDS) for product components are available on request.

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