Cat. No.: VT281925

virotype ASFV 2.0 PCR Kit (96 reactions)

For rapid and highly reliable detection of DNA from African Swine Fever Virus (ASFV) in 60 mins (Availability/distribution: Outside the U.S. and Canada) [Read more]

Log in to see the price

Inquire

This item is not on stock and has to be re-ordered.

African Swine Fever is a threat to animal welfare and one of the most severe diseases affecting swine production. INDICAL’s advanced molecular testing solutions enable you to extract and accurately identify the DNA of the African Swine Fever Virus (ASFV).

Gain more control and confidence in detecting the African Swine Fever Virus from difficult sample material with the new virotype ASFV 2.0 PCR Kit!


Features and specifications

  • Triplex real-time PCR (qPCR) for highly sensitive and specific detection of ASFV DNA in 60 mins
  • Multiple validated sample types (serum, plasma, EDTA blood, tissue and swabs) from pigs and wild boar
  • Suitable for pools of up to 20 samples*
  • Easy-to-use, ready-to-use reagents including a single reaction mix
  • Endogenous Internal Control (β-actin)
  • Exogenous Internal Control (to be added to the Lysis Buffer)
  • Comparable to most common PCR thermocyclers

Why two controls?

Our double control strategy offers a higher level of confidence in the interpretation of results, especially when testing difficult sample material.

  • Control of sample quality and animal origin
  • Control of DNA extraction
  • Detection of PCR (partial) inhibition

Excellent performance

  • Rapid and safe identification of all known ASFV genotypes
  • Performs well compared to the UPL-PCR Method, which is promoted by the European Union Reference Laboratory for African Swine Fever (EURL-ASF) CISIA-INIA
  • Validated by renowned Reference Laboratories (e.g., BE, DK, DE, PL, ES,  LV, and HU)


Supply certainty & exceptional support

  • We have been the trusted supplier of high quality qPCR test systems since 2007 – helping to control animal diseases (e.g., ASFV, CSF, Bluetongue and BVD)
  • High production capacity and safety stocks
  • Excellent technical support by veterinarians and molecular biologists



Combating African Swine Fever

African Swine Fever (ASF) is one of the most important infectious viral diseases of swine of all ages and causes a wide range of clinical signs characterized by a high rate of morbidity and mortality. The disease is notifiable to the World Organization for Animal Health (OIE). The causative agent is a double-stranded DNA virus belonging to the family Asfarviridae, genus Asfivirus. ASF virus can be transmitted by vectors (soft ticks of the genus Ornithodoros) therefore classified as Arbovirus (arthropod-borne virus). INDICAL’s advanced molecular testing solutions enable you to extract and accurately identify the DNA of the African Swine Fever Virus (ASFV).



*Provided that the sample quality is good and that sampling is not swab-based. Pool recommendations and regulations may vary between countries **Data available on file


For up-to-date licensing information and product-specific disclaimers, see the respective handbook or user manual. Assays for veterinary use only. Regulatory requirements vary by country, product may not be available in your geographic area. PCR- and ELISA kits availability/distribution: Outside the U.S. and Canada.


Pathogen type Virus
Product contents Master Mix (includes primers, probes and enzymes), Positive Control, Negative Control, IC-DNA, Handbook
Species Swine
Pathogen investigated African Swine Fever Virus (ASFV)
Technology qPCR
Handbook
Instrukcja obsługi virotype ASFV 2.0 PCR Kit (Lis 2019 r., PL)
Handbook
Kézikönyv virotype ASFV 2.0 PCR Kit (HB 2503, November 2019, HU)
Handbook
Manual virotype AFSV 2.0 PCR Kit (Enero de 2020, ES)
Handbook
Handbuch virotype AFSV 2.0 PCR Kit (Februar 2021, DE)
Scientific Poster
More control and confidence in the detection of ASFV in difficult sample material (August 2019, EN)
Handbook
Handbook virotype ASFV 2.0 PCR Kit (January 2020, EN)
Certificates of Analysis (CoA)

Request a Certificate of Analysis and see the quality control data for your product.

Please contact support@indical.com

Safety Data Sheets

Safety Data Sheets (SDS) for product components are available on request.

Please contact support@indical.com


The virotype ASFV 2.0 PCR Kit has been succesfully validated by renowned Reference Laboratories (e.g. Germany, Spain, Poland, Belgium, USA, Denmark, UK).

IndiMag Pathogen Kit w/o plastics (384 reactions)
(Old name: MagAttract 96 cador Pathogen Kit w/o Plastics) For rapid and automated purification of viral RNA/DNA and bacterial DNA from a wide range of animal samples based on magnetic bead technology. Choose if you own a IndiMag 48/48s or IndiMag 2.
IndiMag Pathogen Kit (384 reactions)
(Old name: MagAttract 96 cador Pathogen Kit). For rapid and automated purification of nucleic acids from a wide range of animal samples based on magnetic bead technology. All-in-one box version: reagents and plasticware included!
virotype ASFV PCR Kit
For detection of DNA from African Swine Fever Virus (ASFV) (Availability/distribution: Outside the U.S. and Canada)
virotype CSFV RT-PCR Kit
For detection of RNA from Classical Swine Fever Virus (Availability/distribution: Outside the U.S. and Canada)
virotype CSFV 2.0 RT-PCR Kit (96 reactions)
For rapid and reliable detection of RNA from Classical Swine Fever Virus (Availability/distribution: Outside the U.S. and Canada)

virotype SBV 2.0 RT-PCR Kit (100 reactions)
For fast, safe and sensitive detection of RNA from Schmallenberg virus (Availability/distribution: Outside the U.S. and Canada)
IndiMag Pathogen KF96 Cartridge
Prefilled reagent cartridges for automated magnetic-bead based extraction of nucleic acids on KingFisher platform
virotype Influenza A RT-PCR Kit (96 reactions)
For detection of RNA from Influenza A Virus (Availability/distribution: Outside the U.S. and Canada)
virotype BVDV RT-PCR Kit (480 reactions)
For rapid and highly reliable identification of RNA from Bovine Viral Diarrhea Virus genotypes 1 and 2 (BVDV-1, BVDV-2) and atypical pestivirus strains (HoBi-like, BVDV-3) in cattle (Availability/distribution: Outside the U.S. and Canada)
bactotype Mastitis HP2+ PCR Kit (96 reactions)
For identification and differentiation of Mycoplasma bovis and Streptococcus agalactiae and identification of other relevant pathogenic Mycoplasma (Availability/distribution: Outside the U.S. and Canada)