Cat. No.: VT280385

virotype BVDV 2.0 RT-PCR Kit
(100 reactions)

For rapid and highly reliable identification of RNA from all Bovine Viral Diarrhea Virus genotypes in cattle (Availability/distribution: Outside the U.S. and Canada) [Read more]

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Veterinary diagnosticians worldwide rely on INDICAL‘s molecular solutions for Bovine Viral Diarrhea Virus (BVDV) control, using it to test millions of calves annually. Building upon 15 years of experience, we have developed the new virotype BVDV 2.0 RT-PCR Kit (FLI-C 112) with increased ease of use and enhanced protocols.

Test with our best – virotype BVDV 2.0 RT-PCR Kit


  • Faster time to result 
    RT-qPCR runtime of ~1 hour*

  • Gold standard sensitivity and specificity 
    Next-generation triplex real-time RT-PCR assay for reliable identification of all known BVDV genotypes
     
  • Additional internal controls for greater confidence 
    Endogenous Internal Control (β-actin) and Exogenous Internal Control

  • Increased ease of use 
    Ready-to-use reagents including a single reaction mix, saving precious lab time

  • Multiple validated sample types 
    Blood, plasma, serum, tissue, milk, and ear tissue from cattle

  • Extended pooling options The high sensitivity of the virotype BVDV 2.0 RT-PCR Kit permits blood samples (whole blood, plasma, serum) and milk samples to be tested in pools consisting of up to 100 individual samples, and ear tissue samples in pools of up to 25 individual samples.**


* See virotype BVDV 2.0 RT-PCR Kit Handbook for detailed information. ** The high sensitivity of the virotype BVDV 2.0 RT-PCR Kit permits blood samples (whole blood, plasma, serum) and milk samples to be tested in pools consisting of up to 100 individual samples, and ear tissue samples in pools of up to 25 individual samples. Pool recommendations and regulations may vary between countries.


For up-to-date licensing information and product-specific disclaimers, see the respective handbook or user manual. Assays for veterinary use only. Regulatory requirements vary by country, product may not be available in your geographic area. PCR and ELISA kits availability/distribution: Outside the U.S. and Canada


Pathogen type Virus
Product type Ready-to-use assay
Species Ruminants
Pathogen investigated Bovine Viral Diarrhea Virus (BVDV)
Technology RT-qPCR
Handbooks
Handbook virotype BVDV 2.0 RT-PCR Kit (July 2022, EN)
Handbooks
Handbuch virotype BVDV 2.0 RT-PCR Kit (Juli 2022, DE)
Handbooks
Instrukcja obsługi virotype BVDV 2.0 RT-PCR Kit (Lipiec 2022, PL)
Handbooks
Manuel virotype BVDV 2.0 RT-PCR Kit (Julliet 2022, FR)
Brochures
Product flyer virotype BVDV 2.0 RT-PCR Kit (November 2022, EN)
Handbooks
Manual virotype BVDV 2.0 RT-PCR Kit (Julio 2022, ES)
Certificates of Analysis (CoA)

Request a Certificate of Analysis and see the quality control data for your product.

Please contact support@indical.com

Safety Data Sheets

Safety Data Sheets (SDS) for product components are available on request.

Please contact support@indical.com

1. Maes R, Bronson-Doherty P, Chumbley M, Herrmann DC, Wise A, Kull-Mason S, Miller V, Schroeder C (2017): Detection of Bovine Viral Diarrhea (BVD) Virus in Ear Skin Tissue Samples: Evaluation of a Combination of QIAGEN MagAttract 96 cador Pathogen Extraction and virotype BVDV RT-PCR-based Amplification, 16th Annual Meeting of the Canadian Animal Health Laboratorians Network (CAHLN), June 4-7, 2017, University of Guelph, Guelph, ON, Canada
2. Bauermann FV, Falkenberg SM, Vander Ley B, Decaro N, Brodersen BW, Harmon A, Hessman B, Flores EF, Ridpath JF (2014): Generation of Calves Persistently Infected with HoBi-Like Pestivirus and Comparison of Methods for Detection of These Persistent Infections, Journal of Clinical Microbiology, Volume 52 No. 11 p. 3845–3852
3. Hoffman B, Depner K, Schirrmeier, H, and Beer, M (2006): A universal heterologous internal control system for duplex real-time RT-PCR assays used in a detection system for pestiviruses. J. of Virological Methods, Volume 136, Issues 1–2, pp.200–209.
4. Fux, R., and Wolf, G. (2012): Transient elimination of circulating bovine viral diarrhoea virus by colostral antibodies inpersistently infected calves: a pitfall for BVDV-eradication programs? Veterinary Microbiology 161 13–19.


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