Cat. No.: VT290005

virotype IHNV/VHSV RT-PCR Kit (100 reactions)

For simultaneous detection and differentiation of RNA from Infectious Hematopoietic Necrosis Virus (IHNV) and Viral Hemorrhagic Septicemia Virus (VHSV) / (Availability/distribution: Outside the U.S. and Canada) [Read more]

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Infectious Hematopoietic Necrosis Virus (IHNV) and Viral Hemorrhagic Septicemia Virus (VHSV) are two of the most serious pathogens affecting salmonid populations with high mortality and effects on production leading to serious economic impacts in the fish industry. Frequently tested together, INDICAL's virotype IHNV/VHSV RT-PCR Kit enables simultaneous detection of RNA from IHNV and VHSV, reducing the number of tests and hands-on time while speeding up time to result and providing confidence in results compared to lab developed tests.  

Features and specifications

  • Triplex real-time RT-PCR Kit for rapid and highly reliable detection of all known IHNV and VHSV genotypes
  • Fast RT-PCR run time ~68 mins
  • Endogenous Internal Control specific for salmonids (β-actin) gives confidence in result interpretation
  • High sensitivity and specificity 
  • Ready-to-use PCR mix in an easy-to-use format, no additional pipetting required
  • Positive and Negative Controls included
  • First and only multiplex IHNV/VHSV RT-PCR Kit approved in Germany
    by the Friedrich-Loeffler-Institut (FLI-C 133)

Infection of fish with IHNV and VHSV is notifiable to the World Organization of Animal Health (WOAH), and various countries and trading areas, like the European Union, have particular legislation in place for the control of the disease (WOAH, 2017c; EU, 2006/88; EU, 2009/177). Furthermore, a differentiation of the two diseases based on clinical and pathological/anatomical symptoms is not possible. Therefore, monitoring and surveillance to detect and identify IHNV and VHSV are legally defined to protect fish cultures.


For up-to-date licensing information and product-specific disclaimers, see the respective handbook or user manual. Assays for veterinary use only. Regulatory requirements vary by country, product may not be available in your geographic area. PCR- and ELISA kits availability/distribution: Outside the U.S. and Canada.


Product contents Master Mix (includes primers, probes and enzymes), Positive Control, Negative Control, Handbook
Pathogen type Virus
Manufacturer INDICAL BIOSCIENCE
Species Salmonids
Pathogen investigated Infectious Hematopoietic Necrosis Virus (IHNV) and Viral Hemorrhagic Septicemia Virus (VHSV)
Technology RT-qPCR
Brochures
Produktflyer IHNV/VHSV RT-PCR Kit (Oktober 2024, DE)
Handbooks
Gebrauchsanweisung virotype IHNV/VHSV RT-PCR Kit (April 2023, DE)
Brochures
Product Flyer IHNV/VHSV RT-PCR Kit (October 2024, EN)
Handbooks
Handbook virotype IHNV/VHSV RT-PCR Kit (April 2023, EN)
Certificates of Analysis (CoA)

Request a Certificate of Analysis and see the quality control data for your product.

Please contact support@indical.com

Safety Data Sheets

Safety Data Sheets (SDS) for product components are available on request.

Please contact support@indical.com

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