virotype BTV pan/3 2.0 RT-PCR Kit
(100 reactions)

The virotype BTV pan/3 2.0 RT-PCR Kit (FLI-C 158, FLI approval pending) from INDICAL enables rapid and reliable detection of Bluetongue Virus (BTV) RNA, and differentiation of European serotype BTV-3 specifically, in samples from cattle, sheep and goats.

Save precious lab time with virotype BTV pan/3 2.0 RT-PCR Kit

• Enhanced, ultra-fast protocol with a total RT-qPCR runtime of ~1 hour*
• Can be performed in one cycler run with other virotype 2.0 assays including virotype 2.0 BTV/pan 8 2.0 RT-PCR Kit
• Easy-to-use single reaction mix with endogenous internal extraction and amplification control
• Ready-to-use positive and negative controls 

Excellent performance 

• Triplex RT-qPCR for Identification of all known BTV serotypes plus separate detection of BTV-3 in one single PCR run
• Highly sensitive detection of as few as 5 copies per sample
• Usable on most common PCR thermocyclers
• High efficiency on all instruments tested

Flexible with multiple validated sample types 

• Ruminant whole blood (preferred with anticoagulants, e.g., EDTA-blood)
  
• Tissue samples (spleen, lymph nodes) from cattle, sheep and goats

Due to the high sensitivity of the test, pools of up to 10 individual blood samples may be analyzed.
However, the optimum pool size depends on the regional prevalence for BTV.**

Supply certainty & exceptional support

• We have been a trusted supplier of high quality qPCR test systems since 2007
• High production capacity and safety stocks
• Excellent technical support

Bluetongue and Bluetongue Virus (BTV)

Bluetongue is an infectious disease of ruminants caused by the Bluetongue Virus (BTV). BTV is a double-stranded RNA virus of the genus Orbivirus of the family Reoviridae.

BTV is widely distributed around the world and primarily affects sheep, cattle, and goats. Additionally, camelids (such as alpacas) and wild ruminants (including deer and stags) can also be affected. The virus is mainly transmitted by mosquitoes of the genus Culicoides (midges), though transmission through contaminated needles is also possible.

Symptoms of Bluetongue include skin changes in the mouth area, a decline in milk yield, a reduced general condition, abortions, fertility problems, and increased mortality in young animals. The characteristic blue coloration of the tongue is rarely observed.

*Depending on PCR-Cycler. See Handbook for detailed information. **Pool recommendations and regulations may vary between countries.