Cat. No.: VT280373

virotype BVDV RT-PCR Kit
(24 reactions)

For rapid and highly reliable identification of RNA from Bovine Viral Diarrhea Virus genotypes 1 and 2 (BVDV-1, BVDV-2) and atypical pestivirus strains (HoBi-like, BVDV-3) in cattle (Availability/distribution: Outside the U.S. and Canada) [Read more]

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Bovine Viral Diarrhea is an economically important infectious disease of cattle.

Veterinary diagnosticians rely on INDICAL‘s molecular workflows for Bovine Viral Diarrhea Virus (BVDV) control, using it to test millions of calves annually.

The virotype BVDV RT-PCR Kit is intended for the detection of BVDV RNA in blood, plasma, serum, milk and ear tissue samples (individual or pooled) from cattle. It contains all the reagents for real-time RT-PCR including a positive and negative control. With this kit, both reverse transcription and PCR are performed in one reaction tube, reducing the risk of contamination. An internal control excludes the possibility of false negative results.

The test kit uses two specific primer/probe combinations: one for pestiviruses yielding FAM™ fluorescence and one for the internal control yielding HEX™ fluorescence.

The high sensitivity of the virotype BVDV RT-PCR Kit permits blood samples (whole blood, plasma, serum) to be tested in pools consisting of up to 50 individual samples, ear tissue samples in pools of up to 25 individual samples, and milk in pools of up to 100 individual samples.

The virotype BVDV RT-PCR Kit is approved in Germany (since 2007), Switzerland (2018), France (2013), Czech Republic (2014) and Belgium (2014) and highly relied upon in BVDV eradication and control programs (e.g., Ireland, Germany, UK).


For up-to-date licensing information and product-specific disclaimers, see the respective handbook or user manual. Assays for veterinary use only. Regulatory requirements vary by country, product may not be available in your geographic area. PCR and ELISA kits availability/distribution: Outside the U.S. and Canada


Product contents PCR Mix (includes primers, probes and internal control), Enzyme Mix, Positive Control, Negative Control, Handbook
Pathogen type Virus
Product type Ready-to-use assay
Species Ruminants
Pathogen investigated Bovine Viral Diarrhea Virus (BVDV)
Technology RT-qPCR
Handbook
Manuel virotype BVDV RT-PCR Kit (Octobre 2018, FR)
Handbook
Handbuch virotype BVDV RT-PCR Kit (Mai 2018, DE)
Handbook
Handbook virotype BVDV RT-PCR Kit (October 2018, EN)
Brochures
Product flyer virotype BVDV RT-PCR Kit (February 2019, EN)
Brochures
Prospectus produit virotype BVDV RT-PCR Kit (Février 2019, FR)
Certificates of Analysis (CoA)

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Safety Data Sheets

Safety Data Sheets (SDS) for product components are available on request.

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1. Maes R, Bronson-Doherty P, Chumbley M, Herrmann DC, Wise A, Kull-Mason S, Miller V, Schroeder C (2017): Detection of Bovine Viral Diarrhea (BVD) Virus in Ear Skin Tissue Samples: Evaluation of a Combination of QIAGEN MagAttract 96 cador Pathogen Extraction and virotype BVDV RT-PCR-based Amplification, 16th Annual Meeting of the Canadian Animal Health Laboratorians Network (CAHLN), June 4-7, 2017, University of Guelph, Guelph, ON, Canada
2. Bauermann FV, Falkenberg SM, Vander Ley B, Decaro N, Brodersen BW, Harmon A, Hessman B, Flores EF, Ridpath JF (2014): Generation of Calves Persistently Infected with HoBi-Like Pestivirus and Comparison of Methods for Detection of These Persistent Infections, Journal of Clinical Microbiology, Volume 52 No. 11 p. 3845–3852
3. Hoffman B, Depner K, Schirrmeier, H, and Beer, M (2006): A universal heterologous internal control system for duplex real-time RT-PCR assays used in a detection system for pestiviruses. J. of Virological Methods, Volume 136, Issues 1–2, pp.200–209.
4. Fux, R., and Wolf, G. (2012): Transient elimination of circulating bovine viral diarrhoea virus by colostral antibodies inpersistently infected calves: a pitfall for BVDV-eradication programs? Veterinary Microbiology 161 13–19.


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